Flow cytometric analysis showed that Th17 (CD4+IL-17+), TFH17 (CD4+CXCR5+ICOS+IL-17+), and B17 (CD19+IL-17+) cells increased with age or T1D in salivary gland cells, peripheral blood mononuclear cells, and splenocytes (Figure 1E and Supplementary Figure 1F). The gene discussed is ICOS; the disease is type 1 diabetes mellitus.