Similarly, signatures of exhausted T cells were enriched in Treg vs MulTreg, including those from dysfunctional CD8 T cells in Melanoma (35) (NES= –1.71, padj=0.02) and, of relevance, functionally exhausted chimeric antigen receptor (CAR) Treg cells (36) (NES= -1.88, padj =0.0003), Figure S5B. The gene discussed is CD8A; the disease is melanoma.