As anticipated, the intra-rectal TNBS treatment in mice also remarkably enhanced MPO activity, which was observed to be higher in the colitis group (0.7267 ± 0.1002 U/mg) compared to non-colitis control (0.02473 ± 0.006562 U/mg) and other probiotic treated groups viz. MTCC 5690 (0.4733 ± 0.2053 U/mg), MTCC 5689 (0.6233 ± 0.05132 U/mg), and NCFM (0.3600 ± 0.1127 U/mg). Here, MPO is linked to colitis.