CCL4 and hepatocellular carcinoma: Also, Thiele et al. (2017) suggested that hepatic Timp1 mRNA expression from WT mice was upregulated in HCC tissue compared with adjacent paired normal tissue when co-treated with diethylnitrosamine and CCL4. In agreement with these studies, our transcriptomic profile showed increased Timp1 expression in Phb1−/−, compared with Phb1+/− (log2 FC = 10.8) (Table 1).