To compare the contribution of their subnuclei in the regulation of fear and anxiety, PKCδ+ neuronal transmission in the CeL or the BNSTov was constitutively inhibited by expression of tetanus toxin light chain (TeNT) to disturb proper local circuit function and investigated its impact on the behaviors related to two distinct negative emotion, fear and anxiety, under the same experimental conditions. The gene discussed is PRKCD; the disease is Anxiety.