The canonical RN3-LTR demonstrated 4.46 ± 0.05% and 23.94 ± 4.45% RFP positive cells in the absence or presence of NL4-3 viral infection, an enhancement of 5.37-folds in gene expression due to the presence of Tat and Rev. R2N3-LTR, in contrast, showed only a small proportion 0.25 ± 0.04% of cells to be RFP positive suggesting barely visible gene expression in the absence of Tat and Rev. In the presence of NL4-3 viral infection, there is a significant enhancement of gene expression with 19.33 ± 4.45% cells being RFP-positive with a 76-folds enhancement in transcription. The gene discussed is TAT; the disease is viral infectious disease.