We also found that differentially hypohydroxymethylated CpGs are enriched in molecular pathways that are frequently connected to childhood brain tumors, particularly those related to WNT signaling and beta-catenin binding, implicating such genes as APC2, WNT4, WNT11, WNT5B, SHH, and BCL9. 5hmC loss in WNT has been associated with other tumors such as melanoma and colorectal cancer [26, 30]. This evidence concerns the gene WNT4 and brain neoplasm.