We dissected and dissociated ASPWT and ASPcKO tumors, seeded dissociated tumor cells to grow as rhabdospheres, and assayed cell growth when incubated with either GDC-0941 (pan-PI3K inhibitor), MK-2206 (AKT inhibitor), everolimus (mTOR inhibitor), or doxorubicin20,24. Here, AKT1 is linked to neoplasm.