We bred the Rosa26tdTomato reporter mouse into our ASPcKOP7cKO model to generate ASTPcKOP7cKO compound mutant mice allowing us to use flow cytometry to purify tumor cells by sorting tdTomato-positive and CD31 (PECAM)-negative (to remove endothelial cells) cells (Supplementary Fig. 8a)20. The gene discussed is PECAM1; the disease is neoplasm.