In order to dissect the similarities and differences between the functions of DDX3X and DDX3Y, we used a cell line we generated to rapidly and efficiently degrade endogenous DDX3 protein X in human male-derived colorectal cancer HCT 116 cells, which show a stable diploid karyotype (Waldman et al. 1995; Natsume et al. 2016). The gene discussed is DDX3Y; the disease is colorectal cancer.