To exam the biological significance of UBE2S in ovarian cancer, we stably transfected A2780 and SKOV3 cells with PLKO.1 (Ctr), UBE2S shRNA1 (sh-1), UBE2S shRNA2 (sh-2), PCMV (Ctr), and PCMV-UBE2S (UBE2S). This evidence concerns the gene UBE2S and ovarian carcinoma.