Regardless of whether there was anti-CD3/CD28 antibody stimulation, IFN-γ, IL-17A, and IL-21 were significantly decreased (P < 0.05), and IL-10 was significantly increased in the chronic colitis + Se-enriched L. acidophilus group compared with the chronic colitis group (P < 0.05). The gene discussed is CD28; the disease is Chronic colitis.