It was found that the activity of U87-TMZ and U251-TMZ was evidently inhibited, the IC50 concentration was evidently decreased (Figures 4B, C), and the apoptosis was induced (Figure 4D), but the cell activity, IC50 concentration and apoptosis of anti- miR-9-5p and si- ABCC1 were evidently reversed, suggesting that miR-9-5p could act in the TMZ resistance mechanism of glioma by regulating ABCC1. This evidence concerns the gene ABCC1 and glioma.