In melanoma, tyrosine phosphorylation and the activation of p190RhoGAP at invadopodia in response to laminin peptide depends on the activation of β1 integrins74; in breast cancer, invadopodia precursor β1 integrins are activated within 3–5 min after EGF stimulation in a Rac3 GTPase-dependent manner42,58. The gene discussed is ARHGAP35; the disease is breast cancer.