When investigating the function of PRMT5 in MM cells, we designed two specific short hairpin RNAs (shRNAs) and successfully knocked down the relative levels of PRMT5 expression in two MM cell lines (NCI-H929 and U266) compared to that in the negative control group as assessed by western blotting and qRT-PCR (Fig. 2A, B).These results were further confirmed by immunofluorescence-targeting PRMT5 in both cell lines (Fig. 2C). The gene discussed is PRMT5; the disease is Miyoshi myopathy.