Using the Cdh5-CreERT2-TdTomato mouse line, we identified five main EC clusters corresponding to the different lung vessel types (Artery, Vein, CapillaryA, CapillaryB, and Lymphatic), previously identified with different EC isolation strategies based on the surface markers CD3116,34 or ICAM2.35 Interestingly, our analysis of publicly available rat21 and human22 PAH scRNA-seq datasets also revealed the presence of these five distinct subpopulations in rat and human PAH lung tissues. This evidence concerns the gene ICAM2 and pulmonary arterial hypertension.