The enriched pathway analysis for 23 differentially expressed EV proteins by Ingenuity pathway analysis were enriched the Insulin Secretion Signaling pathway, IGF-1 Signaling and 14-3-3-mediated signaling, which reported to relate with neurodegenerative diseases, including AD and CTE [27-30] (Table 3). This evidence concerns the gene IGF1 and Alzheimer disease.