SQSTM1 and melanoma: Next, amodiaquine-exposed A-375 and G361 melanoma cells exhibited the formation of multivesicular single membrane-enclosed structures with electron-dense inclusions (indicative of lysosomal expansion), impairment of mitochondrial transmembrane potential, and accumulation of LAMP1, p62/SQSTM1, α-synuclein, lipofuscin, and LC3-II at concentrations as low as 1 μM [57], all accumulating autophagic proteins as a consequence of blocked autophagic-lysosomal flux (Figure 3).