The transcriptional regulation of miRNAs is not fully understood, and we did not find out the transcription factors which regulated the expression of Mir342 and Evl. We mainly investigated the major mature form of Mir342, i.e., miR-342-3p, however, minor mature form of miR-342-5p may have a role in obesity and T2D. Here, EVL is linked to obesity due to melanocortin 4 receptor deficiency.