Comparing the profile of NASH microtissues (PHH, KC, HSC with FFA) with the Human Gene Atlas database (www.ebi.ac.uk/gxa/home) identified a human liver profile (Fig. 2a) and comparison to the DISEASES database (https://diseases.jensenlab.org/Search) produced a close correlation with human fatty liver disease (Fig. 2b). The gene discussed is CALCA; the disease is metabolic dysfunction-associated steatohepatitis.