CHI3L1 and metabolic dysfunction-associated steatohepatitis: These changes were corroborated by measurement of secreted clinical fibrotic markers TIMP-1, pro-collagen 1, YKL-40 and fibronectin, which were all significantly increased in NASH compared to steatotic and control microtissues (Fig. 1c) and the increased expression of genes associated with fibrosis in the NASH microtissues (Fig. 1d and Supp.