To further investigate the relationship between PITX1, SOX9 and SOX10 expression and clinical prognosis, we used RNA-seq datasets (GSE46517, GSE15606, and the cancer genome atlas [TCGA]-SKCM) from the Gene Expression Omnibus (GEO) and TCGA database to measure the expression level of these genes in melanoma primary, metastatic, and normal skin tissue. The gene discussed is SOX9; the disease is melanoma.