In order to evaluate aggregate forming capacity of p53 and its mutants, plasmids expressing WT p53, p53R175H or p53R273H as well as plasmids expressing WT and mutated Δ133p53β isoforms, namely WT Δ133p53β, Δ133p53βR175H and Δ133p53βR273H were transfected into lung cancer H1299 cell line, which is devoid of endogenous p53, thus convenient for the study of individual isoform characteristics. Here, TP53 is linked to lung cancer.