Consistent with the results of our above functional studies, we found that the endogenous circHMGCS1–016 level in ICC patients’ tumor tissues was positively correlated with the level of CD73 (R2 = 0.7379, p < 0.0001) and GAL-8 (R2 = 0.6747, p < 0.0001). This evidence concerns the gene NT5E and neoplasm.