Here, we further determined the GAL-8 in the supernatant of ICC cells with different circHMGCS1–016 or miR-1236-3p expression by ELISA and high concentration of GAL-8 was found in cells overexpressing circHMGCS1–016, while low in cells expressing low level of miR-1236-3p level (Fig. 4F), which was consistent with the supernatant sCD73 level in ICC cells (Fig. 4G). Here, LGALS8 is linked to intrahepatic cholangiocarcinoma.