Immunostaining of GFAP or Iba1 on cerebrum revealed a dramatic upregulation of GFAP and Iba1 in brain regions where sulfatide is present/enriched, including CC and AD relevant brain regions, e.g., the inner cortical layers and partial hippocampus substructure in both CST cKO (Fig. 5B, C) and CST KO mice (Fig. S7A,B). Here, AIF1 is linked to Alzheimer disease.