APOE and Alzheimer disease: To further analyze the resemblance of gene expression profiles with sulfatide deficiency, we first did gene enrichment analysis by inputting 72 DEGs from both CST cKO and KO CNS (Fig. 3C) into GWAS Catalog 2019 screening on Enrichr [62], which revealed that the top related disease was AD (Fig. 4A), with four AD risk genes being significantly upregulated under several conditions of sulfatide deficiency: Apoe, Trem2, Cd33, and Mmp12 (Fig. 4A-E).