In vitro, the results of a co-culture experiment of tumor cells and T cells showed that the knockdown of PRMT5 in tumor cells could enhance the expression of IFN-γ, TNF-α and granzyme B in T cells (Figure S3A), and overexpression of PRMT5 in tumor cells could decrease the expression of these cytokines in T cells (Figure S3B). The gene discussed is TNF; the disease is neoplasm.