Zhou and colleagues discovered that carbonyl cyanide m-chlorophenyl hydrazone (CCCP), a mitochondria-depolarizing agent, can promote iron-mediated ROS generation, which has the potential to trigger GSDME-dependent pyroptosis via the oligomerization of Tom20 (a mitochondrial outer membrane protein), the BAX-induced release of cytochrome C and the activation of caspase-3 in the melanoma cell line A375 and BALB/c nude mice injected with A375 cells 189. The gene discussed is CASP3; the disease is melanoma.