GSDMD and colorectal carcinoma: In the CRC cell line HCT116, which expresses high levels of GSDME but relatively low levels of GSDMD, the combination of TNFα and CHX could induce BAK/BAX, which leads to mitochondrial outer membrane permeabilization (MOMP) and GSDME-mediated pyroptosis through palmitoylation (covalent attachment of fatty acids) at the GSDME C-terminus (GSDME-C)74.