Emerging evidence shows that Val-boroPro can activate pro-caspase-1 and promote the cleavage of GSDMD instead of the apoptotic-related protein PARP through the inhibition of DDP8/DDP9 in the acute myeloid leukemia (AML) cell line MV4;11, which ultimately leads to caspase recruitment domain-containing protein 8 (CARD8)-induced pyroptosis. The gene discussed is CASP1; the disease is acute myeloid leukemia.