PRF1 and neoplasm: Reduced infiltration of CD8+ lymphocytes and NK cells was observed in the microenvironment of the GSDME-/- murine triple-negative breast cancer cell line EMT6 and colorectal cancer cell line CT26; additionally, this reduction was accompanied by the decreased release of granzyme B and perforin by tumor-infiltrating lymphocytes 75 (Figure 4A).