To examine the functional role of CD45 in the organogenesis of APCMin/+ mouse polyps, we isolated single cells from APCMin/+ mouse polyps and silenced CD45 expression using three different siRNAs targeting mPtprc. On day 1, both control- and siPtprc-transfected cells showed a cyst-like morphology in organoid cultures; however, CD45-depleted cells did not generate an epithelium, and consequently, organoids did not grow and ultimately died (Figure 5B-D). This evidence concerns the gene PTPRC and cyst.