First, we collected 30 pairs of HCC tissues and their matched nontumor counterparts and analyzed the expression level of PRMT4 at the mRNA level using qRT-PCR, and the results showed upregulation of PRMT4 in 83.3% (25 of 30) of the HCC specimens compared with their nontumor counterparts (Figure 1A). The gene discussed is CARM1; the disease is hepatocellular carcinoma.