The results of KEGG pathway analysis are provided in Fig. 3C, suggesting HT-DEG-related pathways to be mainly associated with graft-versus-host disease (fold enrichment: 16.54; P value: 4.15E-14), cell adhesion molecules (CAMs) (fold enrichment: 6.66; P value: 4.66E-14), cytokine-cytokine receptor interactions (fold enrichment: 4.94; P value: 5.08E-14), the intestinal immune network for IgA production (fold enrichment: 11.61; P value: 1.24E-11), and the NF-κB signaling pathway (fold enrichment: 6.69; P value: 9.52E-09). The gene discussed is NFKB1; the disease is graft versus host disease.