STAT1 and central nervous system cancer: Due to the feature of alisertib passing through the blood-brain barrier (BBB) 29, we exposed of human U251 cells to alisertib, to assess the generality that inhibition of Aurora-A induced upregulation of STAT1 in glioma, and found that long-term exposure of U251 cells to alisertib also enhanced STAT1 expression along with alisertib resistance (Figures S3A-S3D).