The mRNA levels of IFN-α and IFN-γ, but not IFN-β, were also elevated in alisertib-treated HCT-8 cells, although the levels of IFN-α and IFN-γ were only slightly increased in the serum of the alisertib-treated mice, compared to those of the normal Balb/c nude mice (n = 3, Figure S1C-S1E), indicating that the secreted IFN could bind to their receptors expressing on cancer cells. This evidence concerns the gene IFNG and cancer.