To further understand the relationship between Tspan6 expression and response to treatment in the COIN trial, we carried out reverse stepwise logistic regression with response at 12 wk (response/no response) or overall response (response/no response) as independent variables, with Tspan6 expression, KRAS/BRAF/NRAS/PI3KCA mutation status, microsatellite instability status, age, gender, and tumor site (coded as left/right) as dependent variables. Here, NRAS is linked to neoplasm.