ACTA1 and Hepatic fibrosis: We found that a single dose of rAAV8 could efficiently downregulate the expression of miR-497 in the liver of mice with an injection of CCl4, which increased the target molecule Smad7 and amelioration of liver fibrosis as indicated by the decreased expression of hydroxyproline content, α-SMA, COLI, and COLIII (Fig. 5).