Next, to further verify the role of XBP-1u underlying the biological behavior of KCNQ1OT1/XIST during CRC progression, HCT116 cells were transfected with XBP-1u knockdown vector (KD-XBP-1 tv1#1/2) and the negative control (KD NC). Here, KCNQ1OT1 is linked to colorectal carcinoma.