The IC50 of this DEPTOR mutant was comparable to the wild-type DEPTOR for both the wild-type mTORC1 (Figure 2D) and mTORC1 with an activating cancer-associated mTOR mutation (A1459P, Figure 2—figure supplement 1C), so interaction between these S/T residues and mTORC1 is not important for inhibition by DEPTOR. This evidence concerns the gene DEPTOR and cancer.