To confirm that mineral oil application induced liver inflammation, we compared chronic mineral oil-injected mice with sham operation and found similar amounts of hepatic Cd45 mRNA, the marker of total leukocytes, but the mRNA levels such as Cd11b, F4/80, Ly6G, Mpo and Lcn2, indicators of inflammatory responses, were significantly higher in the wild type receiving mineral oil (Supplementary Fig. 2A). The gene discussed is LCN2; the disease is Hepatitis.