HCCS and cancer: Cancer cells exhibiting a Warburg-like physiology have increased mitochondrial fragmentation compared to cells utilizing oxidative phosphorylation35, therefore we analyzed mitochondrial morphology by generating a transgenic zebrafish line, Tg(actb2:MLS-EGFP), which expressed GFP with the Cox VIII mitochondrial localization sequence (MLS) fused to the N-terminus thereby making the mitochondria fluorescent36.