In line with the previous observations in the Trappc4△IEC CAC models and sh-Trappc4 expressing MC38 tumor models, overexpression of Trappc4 inhibited CD8+ T-cell-mediated antitumor immunity, with less infiltration of CD8+ T cells in tumor areas (Fig. 6e and Supplementary Fig. 8a), reduced T-cell activation marked by CD69+ CD8+ T-cell subsets (Fig. 6f and Supplementary Fig. 8b) and fewer degranulation populations marked by CD107a (Fig. 6g and Supplementary Fig. 8c). Here, CD69 is linked to neoplasm.