IR, defined as the inability of insulin to increase glucose uptake and usage in an individual as much as in healthy population,9 is a long-known major risk factor for the development of T2DM.10 IR accompanied with abnormally increased body fat is a hallmark of pre-diabetes, which has recently been noted as a highly probable major underlying condition for the development of metabolic syndrome.11 Moreover, pre-diabetes accompanied with IR and deteriorated β cells accounts for approximately 5%–10% of patients with newly diagnosed T2DM per year.12 This evidence concerns the gene INS and diabetes mellitus.