SLC2A1 and esophageal squamous cell carcinoma: Subsequently, CCK-8, colony formation and EdU staining was used to assess the proliferation ability of ESCC cells, the results demonstrated that SLC2A1-AS1 downregulation significantly suppressed cell proliferation, reduced colony formation and EdU positive staining cell numbers (Fig. 3B-F), whereas the opposite data was presented after SLC2A1-AS1 overexpression (Supplementary Fig. 3B-F).