Importantly, SLC2A1-AS1 sponged miR-378a-3p to increase the Glut1 expression, further promoted the expressions of glycolysis-related proteins, which led to ESCC progression and increased glycolysis, and thus targeting SLC2A1-AS1/miR-378a-3p/Glut1 signal axis may be a novel therapeutic target for ESCC patients. Here, SLC2A1 is linked to esophageal squamous cell carcinoma.