To explore the possible regulatory factors of SLC2A1-AS1 in ESCC cells, hTFtarget and JASPAR online software were used to predict the binding sites of transcription factors in the promoter region of SLC2A1-AS1, we found that GLI3 potentially bound to three sites of SLC2A1-AS1 promoter region. This evidence concerns the gene GLI3 and esophageal squamous cell carcinoma.