Whether SLC2A1-AS1 affected GLI3 expression in ESCC cells, to this end, we firstly analyzed the possible regulatory correlation of SLC2A1-AS1 by LncACTdb2.0 online software, we found that SLC2A1-AS1 might manipulate the GLI3, VEGFA, RUNX1, etc. (Supplementary Fig. 2A and B), further investigation showed that SLC2A1-AS1 was tightly associated with self-sufficiency in growth signals, evading apoptosis, tissue invasion and metastasis as well as reprogramming energy metabolism (Supplementary Fig. 2C). The gene discussed is GLI3; the disease is esophageal squamous cell carcinoma.