Further Western blot assay showed that SLC2A1-AS1 depletion markedly reduced the expressions of Glut1, HK2, PFKM, PKM and LDHA in EC9706 cells xenografted tumor tissues (Fig. 8F and G); whereas SLC2A1-AS1 overexpression displayed the opposite effects on tumor growth (Fig. 8H and I), mice weight (Fig. 8J), the expressions of SLC2A1-AS1 and miR-378a-3p (Fig. 8K and L) as well as the expressions of glycolysis-related proteins (Glut1, HK2, PFKM, PKM and LDHA) (Fig. 8M and N) in EC9706 cells xenografted tumor tissues. This evidence concerns the gene HK2 and neoplasm.