In this study, we verified that SLC2A1-AS1 downregulation suppressed tumor growth and glycolysis in vitro and in vivo, decreased migration and invasion and enhanced cell apoptosis, coupled with increased E-cadherin expression and reduced expressions of N-cadherin, Vimentin, HK2, PFKM, PKM and LDHA proteins, and converse data were obtained following SLC2A1-AS1 overexpression. This evidence concerns the gene PFKM and neoplasm.