To explore the possible regulatory factors of SLC2A1-AS1 in ESCC cells, hTFtarget and JASPAR online software were used to predict the binding sites of transcription factors in the promoter region of SLC2A1-AS1, we found that GLI3 potentially bound to three sites of SLC2A1-AS1 promoter region. The gene discussed is PTGDR; the disease is esophageal squamous cell carcinoma.