To further verify whether GLI3 expression affected the SLC2A1-AS1 expression in ESCC cells, three GLI3 siRNAs designed were transfected to ESCC cells, we found GLI3 siRNA#1, 2 and 3 significantly downregulated the GLI3 expression in EC9706, TE1 and KYSE180 cells (Fig. 2H), whereas pcDNA3.1-GLI3 markedly upregulated the GLI3 expression in EC9706, TE1 and KYSE180 cells (Fig. 2I). Here, GLI3 is linked to esophageal squamous cell carcinoma.