GLI3 and esophageal squamous cell carcinoma: To verify the precise interaction site of GLI3 in SLC2A1-AS1 promoter region, three mutation vectors harboring different SLC2A1-AS1 promoter mutation region were constructed, these vectors combined with pcDNA3.1 or pcDNA3.1-GLI3 were co-transfected to ESCC cells, we found that the mutation of site 2 significantly reduced the activity of luciferase in EC9706, TE1 and KYSE180 cells (Fig. 2C), suggesting GLI3 mainly binds to the site2 region of SLC2A1-AS1 promoter.