Further investigation revealed that GLI3 bound to the region of SLC2A1-AS1 promoter and promoted its expression in ESCC cells, whereas SLC2A1-AS1 also affected the expression of GLI3, which formed an important regulatory feedback loop in ESCC cells, however, how SLC2A1-AS1 recruited other transcription factors affecting GLI3 expression needs to be further explored. The gene discussed is GLI3; the disease is esophageal squamous cell carcinoma.