SLC2A1 and esophageal squamous cell carcinoma: To investigate how SLC2A1-AS1 exerts its function in ESCC cells, qRT-PCR assay of SLC2A1-AS1 level in the nucleus and cytoplasm demonstrated that SLC2A1-AS1 was mainly localized in the cytoplasm of ESCC cells (Fig. 5A), which was further confirmed by FISH assay (Fig. 5B).