In this article, we further found that the M2 polarization of macrophages co-cultured with TNFSF9 knockdown pancreatic cancer cells was reduced, and this result was dependent on the activation of Wnt signaling by TNFSF9 to promote the release of IL-10 and TGF -β in pancreatic cancer cells. Here, TNFSF9 is linked to pancreatic neoplasm.