Furthermore, we added Wnt agonist to TNFSF9 knockdown pancreatic cancer cells, and found that compared with the sh-TNFSF9 + DMSO group, the expressions of Wnt, β-catenin and Snail were increased in the sh-TNFSF9 + Wnt agonist group (P < 0.05), while the expression of TNFSF9 was not different (Figure 4D, 4E). Here, TNFSF9 is linked to familial pancreatic carcinoma.