And then, we co-cultured TNFSF9-knockdown pancreatic cancer cells with U937-derived macrophages and found that compared with the sh-NC group, the mRNA levels of M1-type markers (TNF-α and IL-8) in sh-TNFSF9-1 and sh -TNFSF9-2 groups were increased and the mRNA levels of IL-1β were decreased. This evidence concerns the gene CXCL8 and familial pancreatic carcinoma.