In addition, at the mRNA and protein levels, the expression of TNFSF9 in pancreatic cancer cells ASPC-1, PANC-1 and BXPC-3 was significantly higher than that of normal pancreatic epithelial cells HPDE6-C7 (P < 0.05), while the expression of COLO357 was not different from HPDE6-C7 (Figure 1D–1F). This evidence concerns the gene TNFSF9 and pancreatic neoplasm.