Consistent with our transcriptome analysis, a recent study by Mournetas et al. (48) also identified down-regulation of genes involved in NMJ formation in DMD patient PSC-derived myotubes (e.g., CHRNA1) and DMD patient-derived primary myoblasts (e.g., CHRNA1 and MUSK), compared to non-isogenic WT controls. This evidence concerns the gene CHRNA1 and Duchenne muscular dystrophy.