PRF1 and neoplasm: Moreover, mRNA expression of effector molecules of NK cells from tumor-infiltrating immune cells from B16-bearing HSD-fed RAG1−/− mice, as compared to B16-bearing ND-fed RAG1−/− mice, showed a robust increase in cytokines (IFNγ and TNFα), cytotoxic factors (perforin and granzyme B), and receptors (CD226 and NCR1) (Fig. 2G).