Western blotting demonstrated that the protein levels of cancer stem cell markers (CD133, Nanog, Oct 4, Sox 2, and ALDH1) in ESCA cells were decreased after transfection with sh-KCNMB2-AS1, but rescued after cotransfection with sh-KCNMB2-AS1 and pcDNA3.1/PYGL (Figure 5(i)). This evidence concerns the gene PROM1 and cancer.