To avoid the possible false positive results from the high- throughput technology, we collected 106 paired HCC tissues/adjacent normal tissues from the local HCC patients, and applied human cell lines (3 from HCC and 1 from normal liver) for qRT-PCR assay to verify the express profile of IGF2-AS obtained by above bioinformatics assay. This evidence concerns the gene IGF2 and hepatocellular carcinoma.