To confirm the essential role of liver‐derived PZP in the anti‐obesity effect, we generated a liver‐specific PZP deficiency model (PZPΔliver) based on adeno‐associated viral (AAV) CRISPR/Cas9 strategy,[45] where sacas9 expression is driven by liver‐specific thyroxine‐binding globulin (TBG) promoter (Figure S7A, Supporting Information). The gene discussed is PZP; the disease is obesity due to melanocortin 4 receptor deficiency.