Since MCD-fed mice recapitulate the histology of human NAFLD (hepatic steatosis, and fibrosis) but lack the metabolic component (obesity, high cholesterol and diabetes) to assess the impact of metabolic imbalances in NLRP3 activation in NASH, ApoE-/- mice were fed with WD. The gene discussed is APOE; the disease is obesity due to melanocortin 4 receptor deficiency.