In conclusion, we suggest that OCT4 and CIP2A play a role in improving the function of testes and isolated Leydig, Sertoli, and germ cells after LPS-induced orchitis, whereas OCT4 overexpression contributes to regulating immune reactions in the testes against the simulated viral infection by acting as a positive modulator of CIP2A during orchitis. Here, POU5F1 is linked to viral infectious disease.