To further explore the differences between the subgroups of 932 CD4+ naive T cells and the functional differences in each subgroup at different tumor sites (Figure 3A and Supplementary Table 4), we found that the CD4+ naive T cells (cluster 0) located at the core of the tumor in the activities of angiogenesis and Wnt-regulating cell proliferation are significantly higher than those at the edge of the tumor; in contrast, the activity in cytokines (such as interferon) and inflammatory response as significantly lower at the core of the tumor than at the edge of the tumor (Figure 3B). This evidence concerns the gene CD4 and neoplasm.