Most of the cells were located in the core and middle of the tumor, which are CD4+ naive T cells (SELL, CCR7, and C1orf162; clusters 0 and 2); regulatory T cells (Tregs; FOXP3, IL2RA, TNFRSF18, and TNFRSF4; cluster 4); CD8+ T cells/natural killer (NK) cells (CD8A, GZMA, GZMB, and NKG7; clusters 1 and 3) and other T cells (unclassified; cluster 6; Figure 2B). The gene discussed is CD8A; the disease is neoplasm.