At 6 and 12 h post-infection, the mRNA expression levels of caspase-3, caspase-1, GSDMD, RIPK3, MLKL, NLRP3, IL-1β, and IL-18 were upregulated in RAW264.7 macrophages infected with either E. faecalis CA1, CA2, or OG1RF strains compared to the levels in the control group. The gene discussed is MLKL; the disease is infection.