At 6 and 12 h post-infection, the mRNA expression levels of caspase-3, caspase-1, GSDMD, RIPK3, MLKL, NLRP3, IL-1β, and IL-18 were upregulated in RAW264.7 macrophages infected with either E. faecalis CA1, CA2, or OG1RF strains compared to the levels in the control group. This evidence concerns the gene GSDMD and infection.