CASP1 and infection: We found that, after 6 and 12 h of real-time infection with viable E. faecalis, macrophages expressed genes and activated proteins (caspase-3 for apoptosis, NLRP3, gasdermin D [GSDMD] and caspase-1 for pyroptosis, and mixed lineage kinase domain-like protein [MLKL] and receptor interacting protein kinase 3 [RIPK3] for Necroptosis) that resulted in simultaneous occurrence of apoptosis, pyroptosis, and necroptosis of the macrophages.