Additionally, DEXs loaded with chaperone-rich cell lysates (CRCLs) derived from GL261 glioma cells promoted proliferation and activity of CD4+ and CD8+ T cells, enhanced T cell infiltration in intracranial glioma tissues, and induced the generation of anti-tumor cytokines, including IL-2 and IFN-γ. This evidence concerns the gene CD8A and central nervous system cancer.