LP-ERN nanoparticles improved the solubility of ERN, and Tf-LP-ERN nanoparticles more effectively targeted tumor cells than LP-ERN nanoparticles, leading to better anti-liver cancer activity in vivo. Consistent with previous studies of ERN, we found that the enhanced anti-liver cancer effects of Tf-LP-ERN were due to immunoregulation. Here, TF is linked to liver cancer.